Epo CLIA Kit for Mouse(Mus musculus) | EIAab.comEpo CLIA Kit for Mouse(Mus musculus)

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Index > Protein center > Epo(Gene name) > Mouse

Epo (Gene name),

Erythropoietin (Protein name ), EPO_MOUSE from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

Epo;

Protein name:

Erythropoietin;

Alternative:

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

N/A

Function:

Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

192

Sequence:

50:

100:

150:

192:

TPPAPLRTLT | VDTFCKLFRV | YANFLRGKLK | LYTGEVCRRG | DR

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

Pfam:

PF00758

UniGene:

Mm.349116

SMR:

P07321

KEGG:

mmu:13856

String:

P07321

Uniprot:

P07321

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ELISA Kit for Mouse Erythropoietin

Cat.:

E0028r

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96T

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ELISA Kit for Mouse Erythropoietin

Cat.:

E0028h

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96T

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ELISA Kit for Mouse Erythropoietin

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E0028d

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ELISA Kit for Mouse Erythropoietin

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E0028p

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ELISA Kit for Mouse Erythropoietin

Cat.:

E0028m

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ELISA Kit for Mouse Erythropoietin

Cat.:

E0028b

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ELISA Kit for Mouse Erythropoietin

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CLIA Kit for Mouse Erythropoietin

Cat.:

U0028b

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96T

CLIA Kit for Mouse Erythropoietin

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CLIA Kit for Mouse Erythropoietin

Cat.:

U0028m

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CLIA Kit for Mouse Erythropoietin

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U0028h

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Looking for manual.

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96T

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CLIA Kit for Mouse Erythropoietin

Cat.:

U0028d

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Looking for manual.

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CLIA Kit for Mouse Erythropoietin

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U0028p

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Looking for manual.

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CLIA Kit for Mouse Erythropoietin

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U0028r

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Polyclonal Antibody for Mouse Erythropoietin

Cat.:

P0028Rb-h

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40ug/0.2ml

Polyclonal Antibody for Mouse Erythropoietin

Cat.:

P0028Rb-r

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40ug/0.2ml

Polyclonal Antibody for Mouse Erythropoietin

Cat.:

P0028Rb-m

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40ug/0.2ml

Polyclonal Antibody for Mouse Erythropoietin

Monoclonal Antibody for Mouse Erythropoietin

Protein for Mouse Erythropoietin

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.1%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of EPO into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	96	94-99
Plasma	98	97-102

Recovery was determined by spiking various levels of EPO into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	91-103
Plasma	98	93-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of EPO into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	95-103
Plasma	98	97-105

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EPO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-100%	90-98%	95-102%	92-97%
EDTA plasma(n=5)	89-95%	93-98%	99-105%	90-97%
heparin plasma(n=5)	93-98%	99-105%	101-107%	96-105%

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-99%	94-98%	95-100%	100-105%
EDTA plasma(n=5)	91-95%	93-99%	89-94%	96-100%
heparin plasma(n=5)	93-96%	99-106%	97-100%	94-102%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-102%	95-101%	98-104%	97-105%
EDTA plasma(n=5)	96-99%	97-104%	100-105%	89-94%
heparin plasma(n=5)	91-96%	95-100%	99-107%	101-109%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Murine erythropoietin gene: cloning, expression, and human gene homology."
Shoemaker C.B. , Mitsock L.D.
Mol. Cell. Biol.6:849-858(1986) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
2.

"Cloning, sequencing, and evolutionary analysis of the mouse erythropoietin gene."
McDonald J.D. , Lin F.-K. , Goldwasser E.
Mol. Cell. Biol.6:842-848(1986) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
3.

"Comparative analysis of the gene-dense ACHE/TFR2 region on human chromosome 7q22 with the orthologous region on mouse chromosome 5."
Wilson M.D. , Riemer C. , Martindale D.W. , Schnupf P. , Boright A.P. , Cheung T.L. , Hardy D.M. , Schwartz S. , Scherer S.W. , Tsui L.-C. , Miller W. , Koop B.F.
Nucleic Acids Res.29:1352-1365(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
strain: 129/Sv.
4.

"Abnormal erythropoietin (Epo) gene expression in the murine erythroleukemia IW32 cells results from a rearrangement between the G-protein beta2 subunit gene and the Epo gene."
Chretien S. , Duprez V. , Maouche L. , Gisselbrecht S. , Mayeux P. , Lacombe C.
Oncogene15:1995-1999(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-52
strain: ICFW.

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